Human TLR7 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC5875P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Immunohistochemistry-Frozen, Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 533707
Product Specifications
Immunogen
E. coli-derived recombinant human TLR7
Met360-Leu516
Accession # Q9NYK1
Met360-Leu516
Accession # Q9NYK1
Specificity
Detects human TLR7 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human TLR7 PE-conjugated Antibody
Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry.
Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PE-conjugated Monoclonal Antibody (Catalog # IC5875P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Human TLR7 by Flow Cytometry
Gating strategy for the detection of TLR-7 and -9 in B cell subsets.PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-7 and -9. FSC and SSC were first used to gate out debris (A) and SSC-A and SSC-H was utilized to eliminate duplicates (B). Further gating was done on CD45 and CD19, to target B cells (C). To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-7 and -9 expression on these subsets (E). Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120383), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human TLR7 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Ramos human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Ramos human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 1 review rated 4 using IC5875P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TLR7
Long Name
Toll-like Receptor 7
Alternate Names
toll-like receptor 7
Gene Symbol
TLR7
UniProt
Additional TLR7 Products
Product Documents for Human TLR7 PE-conjugated Antibody
Product Specific Notices for Human TLR7 PE-conjugated Antibody
For research use only
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