Human TIMP-2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF971
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Antibody Array Development, ELISA Detection
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2 (R&D Systems, Catalog # 971-TM)
Cys27-Pro220
Accession # P16035
Cys27-Pro220
Accession # P16035
Specificity
Detects human TIMP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TIMP‑1, recombinant rat TIMP‑1, recombinant mouse TIMP‑1, and rhTIMP-3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TIMP-2 Biotinylated Antibody
Human TIMP-2 ELISA Standard Curve
Recombinant Human TIMP-2 (Catalog # 971-TM) was serially diluted and captured by Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB9711) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human TIMP-2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human TIMP‑2 Western Blot Standard (Catalog # 971-TM)
Sample: Recombinant Human TIMP‑2 Western Blot Standard (Catalog # 971-TM)
Human TIMP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using BAF971 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-2
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
Long Name
Tissue Inhibitors of Metalloproteinases 2
Alternate Names
TIMP2
Gene Symbol
TIMP2
UniProt
Additional TIMP-2 Products
Product Documents for Human TIMP-2 Biotinylated Antibody
Product Specific Notices for Human TIMP-2 Biotinylated Antibody
For research use only
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