Detects human Thrombopoietin/Tpo in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) G-CSF, rhG-CSF R, rhGM-CSF, recombinant mouse GM-CSF, or rhM-CSF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Thrombopoietin/Tpo Antibody

Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody.

Recombinant Human Thrombo-poietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo-poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo-poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND₅₀ is typically 5-10 µg/mL.

Human Thrombopoietin / Tpo ELISA Standard Curve

Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TPN) was serially diluted and captured by Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Thrombopoietin/Tpo Antibody

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 5‑10 µg/mL in the presence of 1.5 ng/mL Recombinant Human Thrombopoietin/Tpo.

Human Thrombopoietin/Tpo Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog # BAF288)
Detection Reagent: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog # BAM288)
Standard: Recombinant Human Thrombopoietin (NS0-expressed) Protein (Catalog # 288-TPN)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Tpo is a secreted protein that regulates megakaryocytopoiesis and thrombopoiesis. It binds to and induces signaling through the Tpo receptor, which is encoded by the c-Mpl protooncogene.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Gene Symbol

THPO

UniProt

P40225

Additional Thrombopoietin/Tpo Products

Product Documents for Human Thrombopoietin/Tpo Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Thrombopoietin/Tpo Antibody

For research use only

Citations
Reviews

Human Thrombopoietin/Tpo Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB288

Key Product Details

Species Reactivity

Validated:

Cited:

Applications