Human TFPI Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2974AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 374720
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TFPI
Specificity
Detects human TFPI in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse TFPI or recombinant human TFPI-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human TFPI Alexa Fluor™ Plus 680-conjugated Antibody
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: TFPI
Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2).
References
- Bai, H. et al. (2005) Thromb Haemost. 93:1055.
- Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
- Mine, S. et al. (2002) Biochemistry 41:78.
Long Name
Tissue Factor Pathway Inhibitor
Alternate Names
EPI, LACI
Gene Symbol
TFPI
UniProt
Additional TFPI Products
Product Specific Notices for Human TFPI Alexa Fluor™ Plus 680-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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