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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human TCF‑2/HNF‑1β
Gly51-Gln182
Accession # P35680

Specificity

This antibody detects TCF‑2/HNF‑1 beta in direct ELISAs and Western blots. It is expected to recognize all three TCF‑2 isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human TCF-2/HNF-1 beta Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TCF-2/HNF-1 beta

TCF-2, also known as HNF-1 beta (hepatocyte nuclear factor 1 beta) and VHNF-1, is a member of the HNF-1 homeobox family. By alternative splicing, three isoforms of TCF-2/HNF-1 beta exist. TCF-2/HNF-1 beta binds DNA as a homodimer or heterodimer with TCF-1/HNF-1 alpha. Within the region used as the immunogen, human TCF-2 shares 98% amino acid sequence homology with mouse and rat TCF-2. TCF-2 expression is required for visceral endoderm specification.

Long Name

Transcription Factor 2

Alternate Names

HNF-1 beta, HNF1B, HNF2, LF-B3, MODY5, TCF2, VHNF1

Entrez Gene IDs

6928 (Human); 21410 (Mouse); 25640 (Rat)

Gene Symbol

HNF1B

UniProt

Additional TCF-2/HNF-1 beta Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TCF-2/HNF-1 beta Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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