Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SOX18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5077) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX18 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SOX18 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX18

SOX18 (SRY-related HMG box) is a 41 kDa (predicted) Group F member of the SOX family of DNA-binding proteins. Human SOX18 is 384 amino acids (aa) in length and contains an N-terminal DNA-binding HMG box (aa 85‑153), a central TAD region (aa 168‑260) and a highly charged sequence that contains a nine aa transactivation motif (aa 323‑331). SOX18 is expressed by endothelium and vascular smooth muscle, and apparently promotes the formation and organization of new capillaries. Over aa 276‑384, human SOX18 shares 90% and 89% aa identity with mouse and porcine SOX18, respectively.

Long Name

SRY-related HMG-box 18

Alternate Names

HLTS

Entrez Gene IDs

54345 (Human); 20672 (Mouse); 311723 (Rat)

Gene Symbol

SOX18

UniProt

P35713

Additional SOX18 Products

Product Documents for Human SOX18 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SOX18 Antibody

For research use only

Citations
Reviews

Human SOX18 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5077

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SOX18 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SOX18 Antibody

Detection of Human SOX18 by Western Blot.