Detects human Serpin C1/Antithrombin-III in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Serpin C1 is observed and less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin F2, rmSerpin D1, and rhPEDF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Serpin C1/Antithrombin-III Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin C1/Antithrombin-III (Catalog # 1267-PI)

Reviewed Applications

Read 1 review rated 5 using BAF1267 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin C1/Antithrombin-III

Serpin C1 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa (2). Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases (3). For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery (4).

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Chuang, Y.-J. et al. (2001) Biochemistry 40:6670.
Vinazzer, H. (1999) Semin. Thromb. Hemost. 25:257.
Risberg, B. (1998) Blood Coagul. Fibrinolysis Suppl. 3:S3.

Alternate Names

Antithrombin-III, ATIII

Entrez Gene IDs

462 (Human); 11905 (Mouse)

Gene Symbol

SERPINC1

UniProt

P01008

Additional Serpin C1/Antithrombin-III Products

Product Documents for Human Serpin C1/Antithrombin-III Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Serpin C1/Antithrombin-III Biotinylated Antibody

For research use only

Citations
Reviews

Human Serpin C1/Antithrombin-III Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1267

Key Product Details

Species Reactivity

Validated:

Cited:

Applications