Human Semaphorin 4D/CD100 APC-conjugated Antibody

Detection of Semaphorin 4D/CD100 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Semaphorin 4D/CD100 APC-conjugated Monoclonal Antibody (Catalog # FAB74701A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Human Semaphorin 4D/CD100 by Flow Cytometry

Correlation analysis between CD100 expression on B cells and serum HCV-RNA levels during the early phase of IFN-alpha treatment.(A) Representative dot plots of the CD100 percentages in CD5+ and CD5- B cell subsets in one of the six HCV patients are shown. (B) The CD100 expression on CD5+ (open circles) and CD5- B cells (filled circles) and serum HCV-RNA levels (filled rhombs) immediately before and seven days after IFN-alpha /RBV treatment is shown for six chronic HCV-infected patients. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113338), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Semaphorin 4D/CD100 by Flow Cytometry

The effect of IFN-alpha on CD100 expression in B cells and their subpopulations.(A) CD100 expression on CD5+ and CD5- B cells from one representative, healthy donor before and after IFN-alpha stimulation of PBMCs. (B) CD100 expression alterations in B cells and their subsets after 24-h stimulation with 0.01, 0.1, 1, 10, 100 or 1000 ng/ml of IFN-alpha. Two of six representative individuals are shown. (C) CD100 expression at different time points: 0, 2, 6, 12, 24 and 48 h on B cells and their subsets after stimulation with IFN-alpha at a concentration of 0.1 ng/ml (open circles), 10 ng/ml (filled squares) or 100 ng/ml (filled triangles). One of the six representative individuals is shown. (D) The mean fluorescence intensity (MFI) of CD72 expression on B cells stimulated with different concentrations of IFN-alpha. Two of six representative individuals are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113338), licensed under a CC-BY license. Not internally tested by R&D Systems.