Human S1P2/EDG-5 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3649A

R&D Systems, part of Bio-Techne
Discontinued Product
FAB3649A has been discontinued. View all S1P2/EDG-5 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 368510

View all S1P2/EDG-5 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human S1P2/EDG-5
Met1-Val353
Accession # AAC98919

Specificity

Detects human S1P2/EDG-5. Stains human S1P2/EDG-5 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human S1P2/EDG-5 APC-conjugated Antibody

Detection of S1P2/EDG-5 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of S1P2/EDG-5 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human S1P2/EDG-5 APC-conjugated Monoclonal Antibody (Catalog # FAB3649A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P2/EDG-5 antibody in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP antibody by Flow Cytometry.

Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 APC-conjugated Monoclonal Antibody (Catalog # FAB3649A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P2/EDG-5 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HUVEC human umbilical vein endothelial cells and HEK293 human embryonic kidney cell line transfected with human S1P2/EDG-5 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P2/EDG-5

S1P2 (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a 353 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P2 show 82.5% aa identity with mouse S1P2 and 92% aa identity with human EDG-3, the most closely related family member. Both are expressed predominantly in the lung, heart, kidney, liver, spleen, thymus, testis and brain, mediating both mitogenic and anti-apoptotic effects.

Long Name

Endothelial Differentiation Gene 5

Alternate Names

AGR16, EDG5, GPCR13, GPCR18, H218, LPB2, S1PR2

Entrez Gene IDs

9294 (Human); 14739 (Mouse); 29415 (Rat)

Gene Symbol

S1PR2

UniProt

AAC98919

Additional S1P2/EDG-5 Products

Product Documents for Human S1P2/EDG-5 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S1P2/EDG-5 APC-conjugated Antibody

For research use only

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