Human Rex-1/ZFP42 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3598
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Rex‑1/ZFP42
Ser2-Lys150
Accession # NP_777560
Ser2-Lys150
Accession # NP_777560
Specificity
Detects human Rex‑1/ZFP42 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human YYI is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Rex-1/ZFP42 Antibody
Rex‑1/ZFP42 in BG01V Human Stem Cells.
Rex-1/ZFP42 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Sheep Anti-Human Rex-1/ZFP24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Rex‑1/ZFP42 in NTera‑2 Human Cell Line.
Rex-1/ZFP42 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Rex-1/ZFP42 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Rex-1/ZFP42 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Rex-1
Sample: Recombinant Human Rex-1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rex-1/ZFP42
Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity.
Long Name
Zinc Finger Protein 42
Alternate Names
Rex1, ZFP42
Gene Symbol
ZFP42
UniProt
Additional Rex-1/ZFP42 Products
Product Documents for Human Rex-1/ZFP42 Antibody
Product Specific Notices for Human Rex-1/ZFP42 Antibody
For research use only
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