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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 852307

Product Specifications

Immunogen

E. coli-derived recombinant human Prostaglandin I2 Synthase
Arg21-Pro500
Accession # Q16647

Specificity

Detects human Prostaglandin I2 Synthase in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Prostaglandin I2 Synthase

PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50-52 kDa (57 kDa predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules. It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium. Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2). Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS. Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation. It apparently does so by binding to either the IP receptor or PPAR gamma. Human PTGIS is 500 amino acids (aa) in length. It is a single span transmembrane protein that contains an N-terminal transmembrane segment (aa 1-20) coupled to a lengthy 480 aa cytoplasmic region. Over aa 21-500, human PTGIS shares 86% aa sequence identity with mouse PTGIS.

Alternate Names

CYP8, CYP8A1, PGIS, Prostacyclin Synthase, PTGI

Entrez Gene IDs

5740 (Human); 19223 (Mouse); 25527 (Rat)

Gene Symbol

PTGIS

UniProt

Additional Prostaglandin I2 Synthase Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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