Human/Primate BMP-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7571
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 66110
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP-4
Ser293-Arg408
Accession # P12644
Ser293-Arg408
Accession # P12644
Specificity
Detects human and primate BMP-4 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) BMP‑2, rhBMP‑3, rhBMP‑5, rhBMP‑6, or rhBMP‑7 under non-reducing conditions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Primate BMP-4 Antibody
Human BMP-4 ELISA Standard Curve
Recombinant Human BMP-4 (Catalog # 314-BP) was serially diluted and captured by Mouse Anti-Human/Primate BMP-4 Monoclonal Antibody (Catalog # MAB7571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Primate BMP-4 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human/Primate BMP-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human BMP‑4 (Catalog # 314-BP) under non-reducing conditions only
Sample: Recombinant Human BMP‑4 (Catalog # 314-BP) under non-reducing conditions only
Human/Primate BMP-4 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-4
BMP-4 forms homodimers or heterodimers with BMP-2 and signals through receptor complexes composed of type I (primarily ActRIA or BMPR-IA) and type II (primarily ActRIIA or BMPR-II) receptors. BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.
Long Name
Bone Morphogenetic Protein 4
Alternate Names
BMP2B, BMP2B1, BMP4, MCOPS6, OFC11
Gene Symbol
BMP4
UniProt
Additional BMP-4 Products
Product Documents for Human/Primate BMP-4 Antibody
Product Specific Notices for Human/Primate BMP-4 Antibody
For research use only
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