Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-IKKa (S176/S180) Mono-clonal Antibody (Catalog # MAB3768) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-IKKa (S176/S180) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human Phospho-IKK alpha (S176/S180) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK alpha

I kappaB kinase alpha (IKK alpha) is also known as IKBKA, CHUK, and IKK1. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF-kappa B. IKK alpha contains kinase, leucine zipper, and helix-loop-helix domains, and heterodimerizes with IKK beta. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha.

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Entrez Gene IDs

1147 (Human); 12675 (Mouse); 309361 (Rat)

Gene Symbol

CHUK

UniProt

O15111

Additional IKK alpha Products

Product Documents for Human Phospho-IKK alpha (S176/S180) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Phospho-IKK alpha (S176/S180) Antibody

For research use only

Citations
Reviews

Human Phospho-IKK alpha (S176/S180) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3768

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all IKK alpha Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-IKK alpha (S176/S180) Antibody

Detection of Human Phospho-IKK alpha (S176/S180) by Western Blot.