Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 nM estradiol for 60 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-ERa/NR3A1 (S118) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5954) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ERa/NR3A1 (S118) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human Phospho-ER alpha/NR3A1 (S118) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with estradiol

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ER alpha/NR3A1

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65‑70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple co-activators. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Phosphorylation at Ser118 regulates ER alpha's role in transcription and cell growth.

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for Human Phospho-ER alpha/NR3A1 (S118) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Phospho-ER alpha/NR3A1 (S118) Antibody

For research use only

Citations
Reviews

Human Phospho-ER alpha/NR3A1 (S118) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5954

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all ER alpha/NR3A1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-ER alpha/NR3A1 (S118) Antibody

Detection of Human Phospho‑ER alpha/NR3A1 (S118) by Western Blot.