Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Caveolin-1 (Y14) at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-Caveolin-1 (Y14) in MCF‑7 Human Cell Line.

Caveolin-1 phosphorylated at Y14 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-Caveolin-1 (Y14) Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Western Blot

0.2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line treated with Pervanadate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caveolin-1

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.

Alternate Names

CAV1, Caveolin1, MSTP085, VIP21

Entrez Gene IDs

857 (Human); 12389 (Mouse); 25404 (Rat)

Gene Symbol

CAV1

UniProt

Q03135

Additional Caveolin-1 Products

Product Documents for Human Phospho-Caveolin-1 (Y14) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-Caveolin-1 (Y14) Antibody

For research use only

Citations
Reviews

Human Phospho-Caveolin-1 (Y14) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7418

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-Caveolin-1 (Y14) Antibody

Detection of Human Phospho-Caveolin‑1 (Y14) by Western Blot.