Human PDGF R beta APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1263A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # PR7212
Product Specifications
Immunogen
Human skin fibroblast membrane extracts
Specificity
Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF Receptor beta-subunit complexed with
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PDGF R beta APC-conjugated Antibody
Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry.
MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta APC-conjugated Monoclonal Antibody (Catalog # FAB1263A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human PDGF R beta APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: MG‑63 human osteosarcoma cell line
Sample: MG‑63 human osteosarcoma cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDGF R beta
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is a potent PDGF antagonist (4).
References
- Hart et al. (1987) J. Biol. Chem. 262:10780.
- Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
- Seifert et al. (1989) J. Biol. Chem. 264:8771.
- Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Alternate Names
CD140b, PDGFRB
Gene Symbol
PDGFRB
Additional PDGF R beta Products
Product Documents for Human PDGF R beta APC-conjugated Antibody
Product Specific Notices for Human PDGF R beta APC-conjugated Antibody
For research use only
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