Human PAR2 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3949P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 344222
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085
Accession # P55085
Specificity
Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PAR2 PE-conjugated Antibody
Detection of PAR2 in HT‑29 Human Colon Adeno-carcinoma Cell Line by Flow Cytometry.
HT-29 human colon adeno-carcinoma cell line was stained with Mouse Anti-Human PAR2 PE-conjugated Mono-clonal Antibody (Catalog # FAB3949P, filled histogram) or isotype control antibody (IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of PAR2 in PC-3 cells by Flow Cytometry.
PC-3 cells were stained with Mouse Anti-Human PAR2 PE-conjugated Monoclonal Antibody (Catalog # FAB3949P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human PAR2 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line or PC-3 prostate cancer cell line
Sample: HT‑29 human colon adenocarcinoma cell line or PC-3 prostate cancer cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PAR2
Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.
Long Name
Protease-Activated Receptor 2
Alternate Names
CFIIRL1, F2RL1, GPCR11
Gene Symbol
F2RL1
UniProt
Additional PAR2 Products
Product Documents for Human PAR2 PE-conjugated Antibody
Product Specific Notices for Human PAR2 PE-conjugated Antibody
For research use only
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