Human PAR1 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3855A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 731115
Product Specifications
Immunogen
E. coli-derived recombinant human PAR1
Arg27-Thr102, Ser375-Thr425
Accession # P25116
Arg27-Thr102, Ser375-Thr425
Accession # P25116
Specificity
Detects human PAR1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PAR1 APC-conjugated Antibody
Detection of PAR1 in Human Peripheral Blood Platelets by Flow Cytometry.
Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human PAR1 APC-conjugated Monoclonal Antibody (Catalog # FAB3855A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of PAR1 in HT-29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 APC-conjugated Monoclonal Antibody (Catalog # FAB3855A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human PAR1 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood platelets and HT‑29 human colon adenocarcinoma cell line
Sample: Human peripheral blood platelets and HT‑29 human colon adenocarcinoma cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PAR1
Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.
Long Name
Protease-Activated Receptor 1
Alternate Names
Cf2r, F2R, HTR, ThrR, TRGPC
Gene Symbol
F2R
UniProt
Additional PAR1 Products
Product Documents for Human PAR1 APC-conjugated Antibody
Product Specific Notices for Human PAR1 APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...