Species Reactivity:

Human

Immunogen:

E. coli-derived recombinant human p70 S6K
Accession # M60725

Applications:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Label:

Alexa Fluor 405 (Excitation=405 nm, Emission=421 nm)

Specificity:

Detects human p70 S6K and p85 S6K (also known as p70 S6K alpha I), an isoform with 23 extra residues at the N-terminus. Reactivity with beta isoforms of p70 S6K is unknown.

View Full Technical Details for Human p70 S6 Kinase Alexa Fluor® 405-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p70 S6 Kinase

p70 S6 kinase is a Ser/Thr kinase activated by such mitogens as EGF, IGF-I, and insulin. The S6 protein of the 40S ribosomal subunit is a major substrate of p70 S6 kinase, and its phosphorylation upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start site. The activity of p70 S6 kinase is controlled by multiple phosphorylation events, with phosphorylation at T229 by PDK1 and T389 by mTOR most critical for kinase function.

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Alternate Names

EC 2.7.11, EC 2.7.11.1, P70S6K1, PS6K, RPS6KB1, Ribosomal protein S6 kinase I, S6K, S6K-beta-1, S6K1, S6K1p70-S6K 1, STK14A, Serine/threonine-protein kinase 14A, p70 S6 Kinase, p70 S6 kinase alpha, p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70 S6KA, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha, p70-S6K, p70-alpha, ribosomal protein S6 kinase beta-1, ribosomal protein S6 kinase, 70kD, polypeptide 1, ribosomal protein S6 kinase, 70kDa, polypeptide 1, serine/threonine kinase 14 alpha

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Product Documents

Product Datasheets

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Human p70 S6 Kinase Alexa Fluor® 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8962V

Key Product Details