Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Olfactomedin-4/OLFM4 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Olfactomedin-4/OLFM4 in Capan-1 (Positive) and U937 (Negative).

Olfactomedin-4/OLFM4 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line (Positive) and absent in U937 human histiocytic lymphoma cell line (Negative) using Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Olfactomedin-4/OLFM4 Antibody

Application

Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed Capan‑1 human pancreatic adenocarcinoma cell line (Positive) and absent in U937 human histiocytic lymphoma cell line (Negative)

Western Blot

2 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB9218 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olfactomedin-4/OLFM4

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF-stimulated clone-1), GW112 or hOlfD, is a 64 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of proteins. Mature Olfactomedin-4 is a 490 amino acid (aa) protein that is secreted mainly as multimers. Human Olfactomedin-4 is mainly expressed by gastrointestinal stem cells, developing granulocytes, type M4 acute myelogenous leukemias, and intestinal and prostate cancer cells and is upregulated by NF-kB signaling. It is reported to facilitate cell-cell adhesion by binding to lectins and cadherins, and to be anti-apoptotic. Mature human Olfactomedin-4 shares 71% aa sequence identity with mouse and rat Olfactomedin-4.

Alternate Names

GC1, GW112, OlfD, OLFM4, OLM4

Entrez Gene IDs

10562 (Human); 380924 (Mouse); 290409 (Rat)

Gene Symbol

OLFM4

UniProt

Q6UX06

Additional Olfactomedin-4/OLFM4 Products

Product Documents for Human Olfactomedin-4/OLFM4 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Olfactomedin-4/OLFM4 Antibody

For research use only

Citations
Reviews

Human Olfactomedin-4/OLFM4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9218

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Olfactomedin-4/OLFM4 Antibody

Detection of Human Olfactomedin-4/OLFM4 by Western Blot.