Human NKG2H Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6549R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 633810
Product Specifications
Immunogen
Human NKG2H synthetic peptide
HQIKFYICSNRND
Accession # Q07444
HQIKFYICSNRND
Accession # Q07444
Specificity
Detects human NKG2H in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human NKG2H Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: NKG2H
Human NKG2H (NK cell Group 2 isoform H; also NK cell receptor H) is a 38 kDa member of the C-type lectin-like domain superfamily of molecules. It represents an alternative splice form of the NKG2E/KLRC3 gene, one of three related genes on human Ch12 that give rise to five isoform variants (NKG2A & B, C, E & H). NKG2H is present on both NK and CD8+ T cells as a 70 kDa disulfide-linked heterodimer with CD94. It serves as a receptor for HLA-E (Qa-1b in mice), an MHC-I like molecule that largely presents fragments of signal peptides associated with classical MHC-I molecules. When engaged, NKG2H is potentially activating, and perhaps will initiate cytolysis and cytokine production. Alternatively, it has been suggested that NKG2H is involved in monitoring the physiological state of its target cell, presumably through the detection of increased levels of HLA-E. In either case, this activity is likely to be context-dependent, as the related NK2GA and B isoforms are inhibitory in nature, and compete for the same target. NKG2H is a 257 amino acid (aa) type II transmembrane glycoprotein. It contains a 70 aa cytoplasmic segment coupled to a 164 aa extracellular region (aa 94-257) that possesses a 108 aa C-type lectin domain (aa 117-224). NKG2H is the long isoform of the KLRC3 gene (SwissProt #:Q07444). The short form (NKG2E) is 240 aa in length, and diverges from the long form beginning at Val227 where 14 aa substitute for aa 227-257 of the long form. Over aa 241-253, human NKG2H shares no meaningful sequence identity with any other NKG2 isoforms or rodent species orthologs.
Long Name
Natural Killer G2H
Alternate Names
KLRC3
Entrez Gene IDs
3823 (Human)
Gene Symbol
KLRC3
UniProt
Additional NKG2H Products
Product Specific Notices for Human NKG2H Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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