Human Neuronal Pentraxin R/NPTXR Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4414
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry, Western Blot, Simple Western
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pentraxin R/NPTXR
Ala24-Lys499
Accession # O95502
Ala24-Lys499
Accession # O95502
Specificity
Detects human Neuronal Pentraxin R/NPTXR in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) NPTX2 and rhNPTX1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Neuronal Pentraxin R/NPTXR Antibody
Detection of Human Neuronal Pentraxin R/NPTXR by Simple WesternTM.
Simple Western lane view shows lysates of U-87 MG human glioblastoma/astrocytoma cell line and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for Neuronal Pentraxin R/NPTXR at approximately 66 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Neuronal Pentraxin R/NPTXR in Hippocampus.
Neuronal Pentraxin R/NPTXR was detected in immersion fixed paraffin-embedded sections of Hippocampus using Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Neuronal Pentraxin R/NPTXR by Western Blot
NPTX levels are reduced in CSF from individuals with clinically diagnosed AD.(A,B) Western blot assays of NPTX2, NPTX1 and NPTXR in lumbar cerebrospinal fluid (CSF) from age-matched controls and patients with clinically diagnosed AD. AD patients show reduced NPTX2, NPTX1 and NPTXR levels in CSF. Control, n = 36; AD, n = 30. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28440221), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Neuronal Pentraxin R/NPTXR Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Hippocampus
Sample: Immersion fixed paraffin-embedded sections of Hippocampus
Simple Western
20 µg/mL
Sample: U‑87 MG human glioblastoma/astrocytoma cell line and Human hippocampus tissue
Sample: U‑87 MG human glioblastoma/astrocytoma cell line and Human hippocampus tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human Neuronal Pentraxin R/NPTXR
Sample: Recombinant Human Neuronal Pentraxin R/NPTXR
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuronal Pentraxin R/NPTXR
NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65 kDa, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2. It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. Human NPTXR is 499 amino acids (aa) in length. It contains a two aa cytoplasmic tail, a 21 aa transmembrane segment, and a 476 aa extracellular region with one Pentraxin domain (aa 295‑499). One alternate start site exists at Met12, and a 55 kDa soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage. Over aa 24‑499, human NPTXR is 87% aa identical to mouse NPTXR.
Alternate Names
NPR, NPTXR
Gene Symbol
NPTXR
UniProt
Additional Neuronal Pentraxin R/NPTXR Products
Product Documents for Human Neuronal Pentraxin R/NPTXR Antibody
Product Specific Notices for Human Neuronal Pentraxin R/NPTXR Antibody
For research use only
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