Human NCOR2 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7017R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NCOR2
Lys2268-Arg2449
Accession # Q9Y618
Lys2268-Arg2449
Accession # Q9Y618
Specificity
Detects human NCOR2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human NCOR1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human NCOR2 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: NCOR2
NCOR2 (Nuclear receptor Co-Repressor 2; also TRAC-2, SMRT alpha and CTG26) is a 265-275 kDa member of the N-CoR family of molecules. NCOR2 is a transcriptional repressor. It forms a complex with Class I and II HDACs (particularly HDAC3) plus TBL1and GPS2, and interacts with a ligand-independent T3R:RXR heterodimer bound to select gene promoters. The HDACs deacetylate lysines in histones, promoting gene repressor binding. Human NCOR2 is 2525 amino acids (aa) in length. It possesses an N-terminal coiled-coil region (aa 174-215), one DNA-binding SANT domain (aa 427-478), a second coiled-coil region (aa 522-561), and a second SANT domain (aa 610-661). There are poly-Gly, -Gln, -Pro and -Lys motifs, and at least five acetylated lysine residues. There are also at least 31 Ser, three Thr, and one Tyr utilized phosphorylation sites that may regulate complex dissociation. There are also multiple splice variants. One isoform (SMRT tau) contains a deletion of aa 724-740 and 2361-2406; a second isoform (SMRTe) contains only a deletion of aa 724-740; a third isoform (hSMRT) shows a deletion of aa 1034-1041; and a fourth isoform (TRAC-1) possesses an alternative start site at Met 1711 coupled to a deletion of aa 2361-2406. Over aa 2268-2449, human NCOR2 shares 95% aa identity with mouse NCOR2.
Long Name
Nuclear Receptor Co-repressor 2
Alternate Names
CTG26, SMRT, SMRTE, TNRC14, TRAC-1
Gene Symbol
NCOR2
UniProt
Additional NCOR2 Products
Product Specific Notices for Human NCOR2 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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