RSK1/RSK2 was detected in perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human Phospho-RSK1 (S221) and RSK2 (S227) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S221) and RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum)

Western Blot

0.5 µg/mL
Sample: PMA-treated HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: RSK1/RSK2

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Long Name

Ribosomal Protein S6 Kinase II

Additional RSK1/RSK2 Products

Product Documents for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF892

Key Product Details

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Species Reactivity

Validated:

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Applications

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Label

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody

RSK1/RSK2 in Mouse Brain.