Human/Mouse/Rat FoxP3 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC3240A

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IC3240A
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

View all FoxP3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxP3
Gln105-Lys200
Accession # Q9BZS1

Specificity

Detects human FoxP3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxD3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat FoxP3 APC-conjugated Antibody

Detection of FoxP3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of FoxP3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Mouse Anti-Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C) or (B) Mouse Anti-Human IL-2 Ra PE-conjugated Monoclonal Antibody (Catalog # FAB1020P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).
Detection of FoxP3 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat Anti-Mouse IL-2 Ra PE-conjugated Monoclonal Antibody (Catalog # FAB2438P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).

Applications for Human/Mouse/Rat FoxP3 APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and mouse splenocytes.

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human); 20371 (Mouse)

Gene Symbol

FOXP3

UniProt

Q9BZS1

Additional FoxP3 Products

Product Documents for Human/Mouse/Rat FoxP3 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat FoxP3 APC-conjugated Antibody

For research use only

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