Detects human FABP1/L-FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with rhFABP2, -3, -4, -5, -6, -7, or rmFABP9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse/Rat FABP1/L-FABP Antibody

Detection of Human FABP1/L-FABP by Western Blot.

Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/ L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/ L-FABP Monoclonal Antibody (Catalog # MAB29641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human FABP1/L‑FABP by Simple Western^TM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/ L‑FABP Monoclonal Antibody (Catalog # MAB29641) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Applications for Human/Mouse/Rat FABP1/L-FABP Antibody

Application

Recommended Usage

Simple Western

5-10 µg/mL
Sample: Human liver tissue, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue

Western Blot

0.25 µg/mL
Sample: Human liver tissue, human colon tissue, human kidney tissue, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue

Reviewed Applications

Read 3 reviews rated 4.3 using MAB29641 in the following applications:

Microarray (1 Review)
Microarrays (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP), Z-protein and squalene-and sterol-carrier protein (SCP) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

P07148

Additional FABP1/L-FABP Products

Product Documents for Human/Mouse/Rat FABP1/L-FABP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat FABP1/L-FABP Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat FABP1/L-FABP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB29641

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FABP1/L-FABP Antibodies »

Product Specifications

Immunogen

Specificity