Human/Mouse/Rat alpha-Actinin 1 Antibody

Detection of Human, Mouse, and Rat alpha-Actinin 1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat a-Actinin 1 Monoclonal Antibody (Catalog # MAB8279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse alpha-Actinin 1 by Simple Western^TM.

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.5 mg/mL. A specific band was detected for alpha-Actinin 1 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat alpha-Actinin 1 Monoclonal Antibody (Catalog # MAB8279). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human alpha-Actinin 1 by Simple Western

Detection and quantification of SI protein. (Panels A) and (Panels B) show the concentration-dependence of SI and alpha-actinin signals (Panel C) using automated capillary Western blotting of Caco-2/TC7 lysates. (Panel D) shows Caco-2/TC7 cell surface protein fraction purified after biotinylation from cells cultured on 6-well Transwell filters in glucose or sucrose for 21 days and treated on the apical side for the final 3 days with 1.5 mg/mL OLE. (Panel E) shows the total cellular SI, relative to alpha-actinin with data normalised to the glucose control and presented as mean ± SEM with n = 12, analysed in duplicate runs. The size was determined relative to a protein standard ladder for SI in the total lysate relative to alpha-actinin (F), normalised and presented as mean ± SEM with n = 24. * p < 0.05, ** p < 0.01, *** p < 0.001 from ANOVA followed by Tukey’s post-hoc test. There was no change in the alpha-actinin loading control between the groups. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31266155), licensed under a CC-BY license. Not internally tested by R&D Systems.