Human/Mouse PLA2G4AAlexa FluorÂ® 700-conjugated Antibody

Name: Human/Mouse PLA2G4AAlexa FluorÂ® 700-conjugated Antibody
Brand: R&D Systems
SKU: AF6659N

R&D Systems, part of Bio-Techne | Catalog # AF6659N

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Product Datasheet / COA

Key Product Details

Species Reactivity:

Human, Mouse

Label:

Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)

Specificity:

Detects human and mouse PLA2G4A in direct ELISAs and Western blots.

View Full Technical Details for Human/Mouse PLA2G4AAlexa FluorÂ® 700-conjugated Antibody

Technical Data
Citations / Reviews

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: PLA2G4A

PLA2G4A (Phospholipase A2 Group IVA; also cytosolic phospholipase A2 alpha/cPLA2 alpha and lysophospholipase) is an 100-110 kDa member of the cytosolic PLA2 family of enzymes. Expression can be induced in select cell types, including endothelium, smooth muscle, macrophages, PMNs, fibroblasts, mast cells and platelets. Tumor cells often serve as sources for cPLA2 alpha activity. cPLA2 alpha is synthesized in the cytosol where, upon activation, it translocates to sites containing cell membranes, including the Golgi, ER and nuclear membrane. This translocation allows for enzymatic action on membrane phospholipids, generating free arachidonic acid that is converted into proinflammatory eicosanoids. Human cPLA2 alpha is 749 amino acids (aa) in length. It contains a phospholipid-binding and membrane-association C2 domain (aa 1-178), and an overlapping PLA2c domain that demonstrates catalytic activity (aa 140-740). While phosphorylation on Ser505 and Ser727 contribute to activation, there are at least five other utilized phosphorylation sites on the molecule. Phosphorylation may increase the MW of cPLA2 alpha in SDS-Page to about 100 kDa. Full-length (aa 1-749) human PLA2G4A/cPLA2 alpha shares 94% aa sequence identity with mouse PLA2G4A.

Item	Value
Long Name	Phospholipase A2 Group IV A
Entrez Gene IDs	5321 (Human); 18783 (Mouse); 24653 (Rat)
Alternate Names	MGC126350, PLA2G4A, PLA2G4cytosolic phospholipase A2, Phospholipase A2 group IVA, cPLA2, cPLA2-alpha, calcium-dependent phospholipid-binding protein, lysophospholipase, phosphatidylcholine 2-acylhydrolase, phospholipase A2, group IVA (cytosolic, calcium-dependent)

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