Human/Mouse BMP-8b Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6305AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects mouse BMP-8b in direct ELISAs and human and mouse in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) BMP-8a and less than 1% cross‑reactivity with recombinant human (rh) BMP‑3b, rhBMP‑10, and rm BMP‑10 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: BMP-8b
BMP-8b (Bone morphogenetic protein 8b; also OP-3) is a 16 kDa predicted mature and 45 kDa precurser member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399 amino acids (aa) in length. It contains a 19 aa signal sequence, a 241 aa prosegment, and a 139 aa mature region. An interchain disulfide bond occurs at Cys363, generating a homodimer. Over aa 261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b, respectively.
Long Name
Bone Morphogenetic Protein 8b
Alternate Names
OP-3
Gene Symbol
BMP8B
UniProt
Additional BMP-8b Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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