Detects human MMP-10 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP‑3 and rhMMP‑7 is observed under non-reducing conditions. Approximately 10% and 2% cross-reactivity with rhMMP-3 and rhMMP-2 is observed under reducing conditions, respectively. Additionally, less than 1% cross-reactivity with rhMMP-1, -8, -9, -12, -13, and recombinant mouse MMP-9 is observed under reducing and non-reducing conditions.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP-10 Antibody

MMP-10 in Human Placenta.

MMP-10 was detected in immersion fixed paraffin-embedded sections of human placenta using Human MMP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF910) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human Total MMP-10 ELISA Standard Curve

Recombinant Human MMP-10 (Catalog # 910-MP) was serially diluted and captured by Mouse Anti-Human MMP-10 Monoclonal Antibody (Catalog # MAB9103) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF910) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human MMP-10 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus) and placenta

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)

Reviewed Applications

Read 1 review rated 1 using AF910 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-10

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short linker region, and a carboxyl terminal hemopexin-like domain.

Long Name

Matrix Metalloproteinase 10

Alternate Names

MMP10, Stromelysin 2

Entrez Gene IDs

4319 (Human)

Gene Symbol

MMP10

UniProt

P09238

Additional MMP-10 Products

Product Documents for Human MMP-10 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human MMP-10 Antibody

For research use only

Citations
Reviews

Human MMP-10 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF910

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity