Human MICA PE-conjugated Antibody

Detection of MICA in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE-conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

MICA Specificity is Shown by Flow Cytometry in Knockout Cell Line.

MICA knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE-conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the MICA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Detection of MICA by Flow Cytometry

Expression of the Sudan virus (SUDV)- or Makona-GP results in loss of surface staining of alphaMICA in HEK293T cells and JEG3 cells stably transfected with MICA. The figure shows representative flow cytometry analysis for steric shielding of the MICA antigen by SUDV-GP or Makona-GP. (A) HEK293T cells were transfected, harvested, and stained for SUDV-GP with a biotinylated 3C10 antibody, followed by allophycocyanin (APC)-conjugated streptavidin, and co-stained for MICA surface antigens with phycoerythrin (PE)-conjugated alphaMICA antibody. Results are from one representative experiment of more than 10 performed (B) HEK293T cells were transfected, harvested, and stained for Makona-GP with survivor sera, followed by APC-labeled secondary antibody, and co-stained for MICA surface antigen using PE-conjugated alphaMICA antibody. Results are from one representative experiment of two performed. (C) SUDV-GP-transfected JEG3-MICA cells stained for SUDV-GP and co-stained for MICA surface antigens and NKG2D-Ig ligands. Results are from one representative experiment of more than four performed. (D–F) HEK293T cells were transfected with SUDV-GP or SUDV-GP-GFP, harvested shortly after they were treated with trypsin or left untreated, and finally stained for: (D) HLA-A, B, C, and SUDV-GP, (E) MICA and SUDV-GP, and (F) B7H6. Results are from one representative experiment of more than four performed. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30013549), licensed under a CC-BY license. Not internally tested by R&D Systems.