Human Melan-A/MART-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8008

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AF8008-SP
AF8008
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Melan-A/MART-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Melan-A/MART-1
Asn52-Pro118
Accession # Q16655

Specificity

Detects human Melan-A/MART-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Melan-A/MART-1 Antibody

Detection of Human Melan-A/MART-1 antibody by Western Blot.

Detection of Human Melan-A/MART-1 by Western Blot.

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Melan-A/ MART-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Melan-A/MART-1 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Melan-A/MART-1 antibody by Simple WesternTM.

Detection of Human Melan-A/MART-1 by Simple WesternTM.

Simple Western lane view shows lysates of SK-Mel-28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Melan-A/MART-1 at approximately 21 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Melan-A/MART-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8008) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Melan-A/MART-1 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line

Western Blot

0.5 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melan-A/MART-1

Melan-A (MeLANoma-A; also MART-1, LB39-AA and SK29-AA, MLANA) is a 18-24 kDa member of a melanocyte lineage-specific, generally structurally-unrelated family of proteins. It is expressed only in melanocytes, retinal pigment epithelium and melanoma cells. Melan-A is involved in melanosome formation, and appears to stabilize both GPCR143/OA-1 and PMEL, thus ensuring the formation of a stage 2 (melanin production within a fibrillar matrix) melanosome. It is typically found in the Golgi, and upon inversion of its topology, becomes embedded in the ER. It may also appear in endosomes and on the cell surface where, in theory, it is recognizable by a special naïve type of CD8+ T cell that is derived from the thymus. Notably, these cells normally appear to be unresponsive, even in the face of exposure to skin melanocytes during wound healing. Human MLANA is a 118 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 26 aa extracellular region (aa 1-26), and a 71 aa cytoplasmic domain (aa 48-118). There is one utilized phosphorylation site at Ser108, and the molecule is known to undergo acylation, creating a MW of 23-34 kDa in SDS-PAGE. Over aa 52-118, human Melan-A shares 62% aa sequence identity with mouse MLANA.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Entrez Gene IDs

2315 (Human); 77836 (Mouse); 293890 (Rat)

Gene Symbol

MLANA

UniProt

Q16655

Additional Melan-A/MART-1 Products

Product Documents for Human Melan-A/MART-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Melan-A/MART-1 Antibody

For research use only

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