Detects recombinant human (rh)M-CSF in Western blots. In sandwich ELISAs, less than 7% cross-reactivity was observed with rmM-CSF. No significant cross-reactivity or interference was observed with over 75 other cytokines tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human M-CSF Antibody

Human M-CSF ELISA Standard Curve

Recombinant Human M-CSF (Catalog # 216-MC) was serially diluted and captured by Mouse Anti-Human M-CSF Monoclonal Antibody (Catalog # MAB616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF216) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human M-CSF Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human M-CSF (Catalog # 216-MC)
under non-reducing conditions only

Human M-CSF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human M-CSF Biotinylated Antibody (Catalog # BAF216)
Standard: Recombinant Human M-CSF Protein (Catalog # 216-MC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB616 in the following applications:

ELISA (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF

M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Human M-CSF Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human M-CSF Antibody

For research use only

Citations
Reviews

Human M-CSF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB616

Key Product Details

Species Reactivity

Validated:

Cited:

Applications