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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 323803

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 1
Ile25-Ser262
Accession # NP_002248

Specificity

Detects human Kallikrein 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) KLK-3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, -14, rhHGFA, rhFactorVII, rhFactorXI,

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Kallikrein 1

Kallikrein 1 (KLK1), also known as tissue kallikrein, is a member of human tissue kallikrein family (1). The best known physiological function of KLK1 is the cleavage of kininogen to release the vasoactive kinin peptide (bradykinin or lysyl-bradykinin), which regulates vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation (2). In addition, KLK1 may play a role in angiogensis and tumorigenesis (2). Human KLK1 precursor contains a signal peptide (residues1 to 18), a short pro peptide (residues 19 to 24) and a mature chain (residues 25 to 262). The purified rhKLK1 contains the mature chain and the propeptide from human KLK5. After being activated by thermolysin, rhKLK1 is active against a fluorogenic peptide substrate described above.

Alternate Names

KLK1

Entrez Gene IDs

3816 (Human); 16612 (Mouse)

Gene Symbol

KLK1

UniProt

Additional Kallikrein 1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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