Human Integrin beta4/CD104 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4060
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta4/CD104
Asn28-Ser710
Accession # P16144
Asn28-Ser710
Accession # P16144
Specificity
Detects human Integrin beta4/CD104 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant mouse Integrin beta4 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin beta2, rhIntegrin beta3, and rhIntegrin beta5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Integrin beta4/CD104 Antibody
Integrin beta4/CD104 in A431 Human Cell Line.
Integrin beta4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Sheep Anti-Human Integrin beta4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4060) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin beta4/CD104 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Integrin beta4/CD104
Sample: Recombinant Human Integrin beta4/CD104
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 4/CD104
Integrin beta 4 (also known as GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human Integrin beta4 ECD shares 88% sequence identity with mouse Integrin beta4 ECD.
Alternate Names
CD104, ITGB4
Gene Symbol
ITGB4
UniProt
Additional Integrin beta 4/CD104 Products
Product Documents for Human Integrin beta4/CD104 Antibody
Product Specific Notices for Human Integrin beta4/CD104 Antibody
For research use only
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