Human Integrin beta2/CD18 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1730
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Adhesion Blockade, Flow Cytometry, Immunocytochemistry, CyTOF-ready
Cited:
Western Blot, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Functional Assay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta2
Gln23-Asn700
Accession # AAA59490
Gln23-Asn700
Accession # AAA59490
Specificity
Detects human Integrin beta2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Integrin beta2/CD18 Antibody
Integrin beta2/CD18 in Human PBMCs.
Integrin beta2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin beta2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Integrin beta2/CD18 in THP-1 cells (Positive) & absent in RT-4 cells (Negative).
Integrin beta2/CD18 was detected in immersion fixed THP-1 human acute monocytic leukemia cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma (Negative) using Goat Anti-Human Integrin beta2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Integrin beta2/CD18 in THP-1 cells by Flow Cytometry
THP-1 cells were stained with Goat Anti-Human Integrin beta2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin beta2/CD18 Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs), THP-1 human acute monocytic leukemia cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma cells (Negative)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs), THP-1 human acute monocytic leukemia cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma cells (Negative)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 2/CD18
Integrin beta2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alphaD, alphaL (complex also known as LFA‑1), alphaM (Mac-1), or alphaX (CR-4). These complexes are important in adhesion and leukocyte trafficking.
Alternate Names
CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta
Gene Symbol
ITGB2
UniProt
Additional Integrin beta 2/CD18 Products
Product Documents for Human Integrin beta2/CD18 Antibody
Product Specific Notices for Human Integrin beta2/CD18 Antibody
For research use only
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