Human Integrin alphaL/CD11a PerCP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB35951C
Discontinued Product
FAB35951C has been discontinued.
View all Integrin alpha L/CD11a products.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # CR38
Product Specifications
Immunogen
Fibronectin purified human monocytes
Specificity
Detects human Integrin alphaL/CD11a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alpha8, alpha9, alpha10, alpha11, alphaM, alphaV, alphaX, rhIntegrin beta3, rhIntegrin alpha6(x1)+ beta4, alpha7(x2)+ beta1, alphaD+ beta2, alphaE+ beta7, recombinant mouse Integrin alpha2b or alphaL is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Integrin alphaL/CD11a PerCP-conjugated Antibody
Detection of Integrin alphaL/CD11a in Whole Blood Monocytes by Flow Cytometry.
Whole blood monocytes were stained with Mouse Anti-Human Integrin aL/CD11a PerCP-conjugated Monoclonal Antibody (Catalog # FAB35951C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin alphaL/CD11a PerCP-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Whole blood monocytes
Sample: Whole blood monocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: Integrin alpha L/CD11a
Integrin subunit alphaL/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alphaL contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alphaL ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.
Alternate Names
CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21
Gene Symbol
ITGAL
Additional Integrin alpha L/CD11a Products
Product Documents for Human Integrin alphaL/CD11a PerCP-conjugated Antibody
Product Specific Notices for Human Integrin alphaL/CD11a PerCP-conjugated Antibody
For research use only
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