Detects human Integrin alpha4/CD49d in direct ELISA. In a sandwich immunoassay, this antibody detects the human Integrin alpha4 beta1 heterodimer when used with the suggested detection antibody, Mouse Anti-Human Integrin beta1 (Catalog # MAB17784).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human Integrin alpha4/CD49d Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody the human Integrin alpha4 beta1 heterodimer when paired with Mouse Anti-Human Integrin beta1 Monoclonal Antibody (Catalog # MAB17784).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta1 (CD29) or beta7. Integrins alpha4 beta1 and alpha4 beta7 are receptors for fibronectin and VCAM. Integrin alpha4 beta1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Alternate Names

CD49d, ITGA4, VLA-4

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Gene Symbol

ITGA4

UniProt

P13612

Additional Integrin alpha 4/CD49d Products

Product Documents for Human Integrin alpha4/CD49d Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin alpha4/CD49d Antibody

For research use only

Citations
Reviews

Human Integrin alpha4/CD49d Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB13541

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Integrin alpha 4/CD49d Antibodies »

Product Specifications

Immunogen

Specificity