Human IL-13 R alpha1 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1462A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 419718
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-13 R alpha1
Ala27-Thr343
Accession # P78552
Ala27-Thr343
Accession # P78552
Specificity
Detects human IL-13 R alpha1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-13 R alpha1, recombinant human (rh) IL-13 R alpha2, rhIL-4 R, rhIL-5 R beta, or rhIL-9 R is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IL-13 R alpha1 APC-conjugated Antibody
Detection of IL‑13 R alpha1 in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and (B) Mouse Anti-Human IL-13 Ra1 APC-conjugated Monoclonal Antibody (Catalog # FAB1462A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human IL-13 R alpha1 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood granulocytes
Sample: Human peripheral blood granulocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IL-13 R alpha 1
Two type 1 membrane proteins belonging to the hematopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, or NR4, is now referred to as IL-13 R alpha1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha', is now referred to as IL-13 R alpha2.
The human IL-13 R alpha1 was originally cloned based on sequence homology to the mouse IL-13 R alpha1. The IL-13 R alpha1 cDNA encodes a 427 amino acid (aa) precursor protein with a putative 21 aa signal peptide, a 324 aa extracellular domain, a 23 aa transmembrane region and a 59 aa cytoplasmic tail. Human and mouse IL-13 R alpha1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha1 is also closely related to that of IL-13 R alpha2. IL‑13 R alpha1 has been shown to combine with the IL‑4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha2 in IL-13 signaling remains to be elucidated.
Long Name
Interleukin 13 Receptor alpha 1
Alternate Names
CD213a1, IL-13Ra1, IL13R alpha 1, IL13RA1
Gene Symbol
IL13RA1
UniProt
Additional IL-13 R alpha 1 Products
Product Documents for Human IL-13 R alpha1 APC-conjugated Antibody
Product Specific Notices for Human IL-13 R alpha1 APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...