Human IgM Antibody

IgM in Human Non-Hodgkins Lymphoma via seqIF™ staining on COMET™

IgM was detected in immersion fixed paraffin-embedded sections of human Non-Hodgkins Lymphoma using Rabbit Anti-Human IgM, Monoclonal Antibody (Catalog #MAB9435) at 0.2ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​​

Detection of Human IgM by Western Blot.

Western blot shows lysates of human serum, monkey serum, mouse serum and rat plasma. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for IgM at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human IgM by Simple Western^TM.

Simple Western lane view shows lysates of human serum, loaded at 0.2 mg/mL. A specific band was detected for IgM at approximately 100 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.