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Human IFN-beta Antibody Best Seller
Catalog # AF814 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Summary for Human IFN-beta Antibody
Immunogen
E. coli-derived recombinant human IFN-beta
Specificity
Detects human IFN-beta in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IFN-beta Antibody
IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-beta Antibody.
Recombinant Human IFN-beta (8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF814). The ND50 is typically 0.100 - 1.00 µg/mL.Applications for Human IFN-beta Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human IFN-beta
Sample: Recombinant Human IFN-beta
Neutralization
Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.100 - 1.00 µg/mL in the presence of 50 pg/mL Recombinant Human IFN‑ beta.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-beta
IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.
Product Documents for Human IFN-beta Antibody
Product Specific Notices for Human IFN-beta Antibody
Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.
For research use only
Citations for Human IFN-beta Antibody (7)
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Citations are publications that use Bio-Techne products. Selected citations for Human IFN-beta Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: NeutralizationLE Langbein et al. (2022-11-11), Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC Data in Brief, 2022-145(0):108743.
PMID: 36426057 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationD Olmeda et al. (2021-11-11), Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021-10(0):e12924.
PMID: 34762341 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationPopugaeva E et al. (2012-04-24), Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. PLoS ONE, 2012-07(4):e35587.
PMID: 22545121 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationKnoblach T et al. (2011-04-14), Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma. PLoS Pathog., 2011-07(4):e1002016.
PMID: 21533215 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationRascle A et al. (2008-10-28), The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes. Exp. Cell Res., 2008-1315(1):76-96.
PMID: 18996370 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationSalaun B et al. (2006-04-15), TLR3 can directly trigger apoptosis in human cancer cells. J. Immunol., 2006-0176(8):4894-901.
PMID: 16585585 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationEmori Y et al. (2004-09-01), Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages. J. Gen. Virol., 2004-085(0):2603-13.
PMID: 15302954
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