Human ICAM-3/CD50 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF813
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Western Blot, ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ICAM‑3/CD50
Gln30-His485
Accession # CAA49473
Gln30-His485
Accession # CAA49473
Specificity
Detects human ICAM‑3/CD50 in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human ICAM-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ICAM-3/CD50 Antibody
Human ICAM-3 / CD50 ELISA Standard Curve
Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC) was serially diluted and captured by Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # BBA15) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM-3/CD50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF813) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human ICAM-3/CD50 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-3/CD50
Intercellular Adhesion Molecule-3 (ICAM-3; also known as CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alphad/ beta2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.
References
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Entrez Gene IDs
3385 (Human)
Gene Symbol
ICAM3
UniProt
Additional ICAM-3/CD50 Products
Product Documents for Human ICAM-3/CD50 Antibody
Product Specific Notices for Human ICAM-3/CD50 Antibody
For research use only
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