Human HNF-4 alpha/NR2A1 Antibody

Detection of HNF-4 alpha/NR2A1 by Flow Cytometry

DKK2 is required for Lgr5 positive stem cells-driven liver metastasis of colorectal cancer.(A) Isolated cells from AKP tumor organoids expressing the tdTomato reporter gene were dissociated and injected into the spleen of wild-type C57BL/6 mice. 8 weeks after the injection, metastatic tumor growth was measured by in vivo imaging analysis. (B) Representative pictures of in vivo imaging analysis. Ctrl: AKP control organoids transduced with scrambled guide RNA, KO: Dkk2 knockout AKP organoids. (C–D) Statistic analysis of liver metastasis (C) and survival (D). Ctrl (n=10), KO (n=15), n represents the number of mice. c.p.m. in (C): count per minute. (E) Quantitative gene expression analysis of Dkk2, Lgr5, and Hnf4 alpha1 in liver metastasized colon cancer cells. Ctrl (n=11), KO (n=12), n represents the number of isolated cancer nodules with five mice per group. (F) Representative flow cytometry analysis data of c-Src phosphorylation (pSrc) and Lgr5 expression in metastasized colon cancer cells. (G) Statistic analysis of Lgr5 expression and pSrc in (E). The average of mean fluorescence intensity in control samples was set as 1 and relative fluorescence intensity was calculated. (H) Statistic analysis of the percentile of Lgr5 high (Lgr5 and pSrc double positive) cells in metastasized colon cancer in (F). Each symbol represents an individually isolated cancer nodule. n.s.=not significant, **p<0.01, ****p<0.0001; two-tailed Welch’s t-test (C, E, G, H). Error bars indicate mean ± s.d. Log-rank test (D). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39535280), licensed under a CC-BY license. Not internally tested by R&D Systems.