Human HLA-E Alexa Fluor™ Plus 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB11547AFP647

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FAB11547AFP647-100UG
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 1079755

View all HLA-E Antibodies »

Product Specifications

Specificity

Detects a synthetic peptide around aa 80 in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human HLA-E Alexa Fluor™ Plus 647-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Background: HLA-E

HLA-E is a class I histocompatibility antigen involved in immune self-nonself discrimination. HLA-E classically binds epitopes from HLA-A, HLA-B, HLA-C and HLA-G signal peptides and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors on NK and t-cell subsets.  HLA-E down-regulates NK cell cytolytic activity when presented on the cell surface.  It can also restrict non-canonical CD8+ T cells during natural infection with pathogens. HLA-E protein expression in normal human nonlymphoid organs is restricted to endothelial cells.  It is also basally expressed by B and T-lymphocytes, NK cells and macrophages.  TNF-alpha, IL-1beta and IFN-gamma up-regulated cell-surface expression of HLA-EE.

References

  1. Sharpe HR, Bowyer G, Brackenridge S, Lambe T. HLA-E: exploiting pathogen-host interactions for vaccine development. Clin Exp Immunol. 2019 May;196(2):167-177. doi: 10.1111/cei.13292. Epub 2019 Apr 9. PMID: 30968409; PMCID: PMC6468186. 
  2. Lin Z, Bashirova AA, Viard M, Garner L, Quastel M, Beiersdorfer M, Kasprzak WK, Akdag M, Yuki Y, Ojeda P, Das S, Andresson T, Naranbhai V, Horowitz A, McMichael AJ, Hoelzemer A, Gillespie GM, Garcia-Beltran WF, Carrington M. HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses. Nat Immunol. 2023 Jul;24(7):1087-1097. doi: 10.1038/s41590-023-01523-z. Epub 2023 Jun 1. PMID: 37264229; PMCID: PMC10690437. 
  3. Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood. 2007 Apr 1;109(7):2806-14. doi: 10.1182/blood-2006-06-030213. PMID: 17179229.

Long Name

Major Histocompatibility Complex, Class I, E

Alternate Names

HLA-6.2, HLAE, QA1

Entrez Gene IDs

3133 (Human)

Gene Symbol

HLA-E

UniProt

P13747 (Human)

Additional HLA-E Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HLA-E Alexa Fluor™ Plus 647-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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