Recombinant Human Hepcidin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB8307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Hepcidin Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB8307).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Hepcidin DuoSet ELISA Kit (Catalog # DY8307-05) for convenient development of a sandwich ELISA or the Human Hepcidin Quantikine ELISA Kit (Catalog # DHP250) for a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepcidin

Hepcidin, also known as Liver Expressed Antimicrobial Protein 1 (LEAP-1), is a peptide hormone that is involved in the regulation of iron metabolism (1, 2). It is synthesized as a preprohormone that is cleaved intracellularly and secreted as a mature 25 amino acid peptide (1, 3, 4). Hepcidin contains eight cysteine residues that form four disulfide bonds which appear to be important for stability in biological fluids (5). It is predominantly expressed, processed, and secreted by hepatocytes (2, 6). Hepcidin expression is positively regulated by inflammation via IL-6/JAK2/ STAT3 signaling, endoplasmic reticulum stress, and BMP-6 (7-11). BMP-6-dependent Hepcidin induction involves RGM-C/Hemojuvelin, which acts as a co-receptor for BMP-6 (11-13). Conversely, Hepcidin expression is negatively regulated by MMP-15/MT2-MMP and multiple erythropoietic stimuli, including anemia, hypoxia, and Erythropoietin (14-18). MMP-15 downregulates Hepcidin expression by interacting with and cleaving RGM-C (19). Hepcidin was originally identified in human blood and urine as an antimicrobial peptide (1, 3). It has since been shown to regulate iron metabolism. Hepcidin binds the cellular iron exporter Ferroportin, and this interaction results in Ubiquitin-mediated degradation of both Hepcidin and Ferroportin (20-22). Degradation of Ferroportin results in reduced iron release from macrophages, hepatocytes, and duodenal enterocytes, suggesting that Hepcidin may be an effector of inflammatory hypoferremia (20).

Long Name

Hepcidin Antimicrobial Peptide

Alternate Names

HAMP, HEPC, HFE2B, LEAP1, PLTR

Entrez Gene IDs

57817 (Human); 84506 (Mouse); 84604 (Rat)

Gene Symbol

HAMP

UniProt

P81172

Additional Hepcidin Products

Product Documents for Human Hepcidin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Hepcidin Antibody

For research use only

Citations
Reviews

Human Hepcidin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB83071

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Hepcidin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Hepcidin Antibody

Human Hepcidin ELISA Standard Curve.