Human Granzyme B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB29061
Recombinant Monoclonal Antibody.
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2103A
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144
Gly19-Tyr247
Accession # P10144
Specificity
Detects human Granzyme B in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Granzyme B Antibody
Detection of Granzyme B by Western Blot.
Western blot shows lysates of NK-92 human natural killer lymphoma cell line and NK-RL human natural killer lymphocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB29061) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Granzyme B at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Granzyme B by Western Blot.
Western blot shows lysates of NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB29061) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Granzyme B at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human Granzyme B by Simple WesternTM.
Left: Simple Western lane view shows lysates of NK-92 human natural killer lymphoma cell line, loaded at 0.1 mg/ml. A specific band was detected for Granzyme B at approximately 42 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB29061) followed by HRP-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB29061) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the NK-92 human natural killer lymphoma cell line.Applications for Human Granzyme B Antibody
Application
Recommended Usage
Simple Western
2-10 µg/mL
Sample: NK-92 human natural killer lymphoma cell line
Sample: NK-92 human natural killer lymphoma cell line
Western Blot
0.5 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line and NK‑RL human natural killer lymphocytic leukemia cell line
Sample: NK‑92 human natural killer lymphoma cell line and NK‑RL human natural killer lymphocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme B
Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).
References
- Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1549.
- Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
- Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
- Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
UniProt
Additional Granzyme B Products
Product Documents for Human Granzyme B Antibody
Product Specific Notices for Human Granzyme B Antibody
For research use only
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