Human GPRC5D APC-conjugated Antibody

Name: Human GPRC5D APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB6300A

R&D Systems, part of Bio-Techne | Catalog # FAB6300A

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Product Datasheet / COA / SDS

Discontinued Product

FAB6300A has been discontinued. View all GPRC5D products.

Key Product Details

Species Reactivity:

Human

Immunogen:

NS0 mouse myeloma cell line transfected with human GPRC5D

Applications:

Flow Cytometry

Label:

Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)

Source:

Monoclonal Mouse IgG_2B Clone # 571961

Specificity:

Stains human GPRC5D transfectants but not the parental cell line in flow cytometry.

View Full Technical Details for Human GPRC5D APC-conjugated Antibody

Technical Data
Citations / Reviews

Applications for Human GPRC5D APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human GPRC5D APC-conjugated Antibody

	Flow Cytometry Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPRC5D and eGFP was stained with either (A) Mouse Anti-Human GPRC5D APC-conjugated Monoclonal Antibody (Catalog # FAB6300A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPRC5D

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.

Item	Value
Long Name	G Protein-coupled Receptor, Family D, Group 5, Member D
Entrez Gene IDs	55507 (Human); 93746 (Mouse); 500349 (Rat)
Alternate Names	G protein-coupled receptor, family C, group 5, member D, G-protein coupled receptor family C group 5 member D, GPRC5D, MGC129713, MGC129714, orphan G-protein coupled receptor

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