Detects human GLI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human GLI-2 Antibody

Detection of GLI‑2-regulated Genes by Chromatin Immunoprecipitation.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3526) or control antibody (Catalog # 5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # BAF016).Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR.

Applications for Human GLI-2 Antibody

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 10⁶ cells
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, bcl-2 promoter detected by standard PCR

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin, basal cell carcinoma, and prostate cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human GLI-2

Reviewed Applications

Read 3 reviews rated 3.7 using AF3526 in the following applications:

ELISA (1 Review)
Microarray (1 Review)
Microarrays (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-2

GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology.

Long Name

GLI-Kruppel family member GLI2

Alternate Names

GLI2, THP2

Entrez Gene IDs

2736 (Human); 14633 (Mouse); 304729 (Rat)

Gene Symbol

GLI2

UniProt

BAA25668

Additional GLI-2 Products

Product Documents for Human GLI-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GLI-2 Antibody

For research use only

Citations
Reviews

Human GLI-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3526

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications