Recombinant Human GIT1 protein was serially diluted 2-fold and captured by Mouse Anti-Human/Mouse/Rat GIT1 Monoclonal Antibody (Catalog # MAB8508) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GIT1 Monoclonal Antibody (Catalog # MAB85081) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human GIT1 Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human/Mouse/Rat GIT1 Monoclonal Antibody (Catalog # MAB8508). In sandwich ELISAs, this antibody is specific for human GIT1 when paired with the suggested capture antibody.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human GIT1 DuoSet ELISA Kit (Catalog # DY8485-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIT1

GIT1 (ARF GTPase-activating protein GIT1) is a 95 kDa protein that belongs to ADP ribosylation factor family and is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts directly with GIT1 causing enhanced htt proteolysis, indicating that GIT1 distribution and function may contribute to Huntington disease pathology. Within amino acids (aa) 485-636, human and mouse GIT1 share 93% aa sequence identity.

Long Name

G Protein-Coupled Receptor Kinase Interacting ArfGAP 1

Alternate Names

ARF GAP GIT1

Entrez Gene IDs

28964 (Human); 216963 (Mouse); 83709 (Rat)

Gene Symbol

GIT1

UniProt

Q9Y2X7

Additional GIT1 Products

Product Documents for Human GIT1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GIT1 Antibody

For research use only

Citations
Reviews

Human GIT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB85081

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GIT1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GIT1 Antibody

Human GIT1 ELISA Standard Curve.