Human Galectin-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11542
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 194801
Product Specifications
Immunogen
E. coli-derived recombinant human Galectin-3
Ala2-Ile250
Accession # P17931
Ala2-Ile250
Accession # P17931
Specificity
Detects human Galectin-3 in direct ELISAs.
In direct ELISAs, 100% cross-reactivity
with recombinant mouse Galectin-3 is observed and no cross-reactivity with
recombinant human (rh) Galectin-8 or rhGalectin-9 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Galectin-3 Antibody
Detection of Human Galectin-3 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11542) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Galectin-3 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11542, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human Galectin-3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/mL
Sample: Human peripheral blood monocytes fixed with paraformaldehyde and permeabilized with saponin
Sample: Human peripheral blood monocytes fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.2 µg/mL
Sample: Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line
Sample: Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line
Reviewed Applications
Read 1 review rated 4 using MAB11542 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3
Alternate Names
AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2
Gene Symbol
LGALS3
UniProt
Additional Galectin-3 Products
Product Documents for Human Galectin-3 Antibody
Product Specific Notices for Human Galectin-3 Antibody
For research use only
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